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mouse monoclonal primary antibodies for osteocalcin  (R&D Systems)


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    R&D Systems mouse monoclonal primary antibodies for osteocalcin
    Fig. 5. Photomicrographs depicting protein expression for <t>osteocalcin</t> following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse <t>monoclonal</t> osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).
    Mouse Monoclonal Primary Antibodies For Osteocalcin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+osteocalcin+primary+antibody/Human%2FRat+Osteocalcin+Antibody/pm36632355-63-9-16
    Average 95 stars, based on 84 article reviews
    mouse monoclonal primary antibodies for osteocalcin - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Role of cartilage and bone matrix regulation in early equine osteochondrosis."

    Article Title: Role of cartilage and bone matrix regulation in early equine osteochondrosis.

    Journal: Bone reports

    doi: 10.1016/j.bonr.2023.101653

    Fig. 5. Photomicrographs depicting protein expression for osteocalcin following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse monoclonal osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).
    Figure Legend Snippet: Fig. 5. Photomicrographs depicting protein expression for osteocalcin following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse monoclonal osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).

    Techniques Used: Expressing, Immunohistochemical staining, Negative Control

    Related Articles

    Immunohistochemistry:

    Article Title: The use of heparin/polycation coacervate sustain release system to compare the bone regenerative potentials of 5 BMPs using a critical sized calvarial bone defect model
    Article Snippet: Von Kossa staining was performed using an online protocol ( http://www.ihcworld.com/_protocols/special_stains/von_kossa.htm ) to verify pellet mineralization. .. Osteocalcin immunohistochemistry using a mouse anti-human osteocalcin primary antibody (MAB1419, 1:100, R&D Systems) was also performed, as previously described [ 27 ]. .. The diaminobenzidine (DAB) color reaction (Vector Laboratory) was used to reveal osteogenic differentiation, as indicated by a brown color.

    Article Title: The use of heparin/polycation coacervate sustain release system to compare the bone regenerative potentials of 5 BMPs using a critical sized calvarial bone defect model.
    Article Snippet: Von Kossa staining was performed using an online protocol (http://www.ihcworld.com/_protocols/spec ial_stains/von_kossa.htm) to verify pellet mineralization. .. Osteocalcin immunohistochemistry using a mouse anti-human osteocalcin primary antibody (MAB1419, 1:100, R&D Systems) was also performed, as previously described [27]. .. The diaminobenzidine (DAB) color reaction (Vector Laboratory) was used to reveal osteogenic differentiation, as indicated by a brown color.

    Article Title: Influences of donor and host age on human muscle-derived stem cell-mediated bone regeneration
    Article Snippet: Von Kossa staining was performed using an online protocol ( http://www.ihcworld.com/_protocols/special_stains/von_kossa.htm ) to verify pellet mineralization. .. Osteocalcin immunohistochemistry using a mouse anti-human osteocalcin primary antibody (MAB1419,1:100, R&D Systems) was also performed, as previously reported [ ]. ..



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    mouse monoclonal primary antibodies for osteocalcin  (R&D Systems)
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    R&D Systems mouse monoclonal primary antibodies for osteocalcin
    Fig. 5. Photomicrographs depicting protein expression for <t>osteocalcin</t> following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse <t>monoclonal</t> osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).
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    anti human osteocalcin purified mouse monoclonal igg primary antibody  (R&D Systems)
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    Fig. 5. Photomicrographs depicting protein expression for <t>osteocalcin</t> following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse <t>monoclonal</t> osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).
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    mouse anti human osteocalcin primary antibody  (R&D Systems)
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    Effects of 5 different BMPs on the osteogenic differentiation of hMDSCs at day 28 (3D pellet culture). A. MicroCT 3D images showed that the mineralized pellets in 5 BMPs groups were larger than the CTL group. Scale bar=1mm. B. Quantification of mineralized pellet volume. All BMPs groups showed higher mineralized pellet volume compared to the CTL group. The mineralized pellet volume of the BMP2 group was significantly higher than the BMP6 and BMP7 groups. *P<0.05, ** P<0.01, ****, P<0.0001. C. Mineralized pellet density quantification. No statistical difference between BMP groups and the CTL group was found. D. Von Kossa staining. Brown-black color indicates mineralization. All pellets have regions peeled off due to high mineralization in the white region of pellets. E. Immunohistochemistry of <t>osteocalcin</t> for osteogenic pellets. Brown color indicates osteocalcin expression. The mineralized parts of the pellets demonstrate crystal purple color intermingled with osteocalcin brown staining. Scale bars=100μm.
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    mouse anti-human primary antibodies osteocalcine  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology mouse anti-human primary antibodies osteocalcine
    Levels of <t>osteopontin</t> (OP) secreted in the culture medium by osteoblasts cultured on the implants’ surface. A . in medium wih fetal calf serum (FCS), collected on day 8, 14, 21 and 28 (analysis with two-way ANOVA Bonferroni posttest *p < 0.05; **p < 0.01; ***p < 0.001); B . in serum-free medium (collected on day 21 and 28), in simple (OS) and in complex (OC) differentiation mediums (collected on day 35). Legend: cells cultivated in plastic dishes (ctrl), non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ).
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    Image Search Results


    Fig. 5. Photomicrographs depicting protein expression for osteocalcin following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse monoclonal osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).

    Journal: Bone reports

    Article Title: Role of cartilage and bone matrix regulation in early equine osteochondrosis.

    doi: 10.1016/j.bonr.2023.101653

    Figure Lengend Snippet: Fig. 5. Photomicrographs depicting protein expression for osteocalcin following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse monoclonal osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).

    Article Snippet: Immunohistochemistry (IHC) was performed on paraffin-embedded osteochondral sections using mouse monoclonal primary antibodies for Osteocalcin (cat#MAB1419, R&D Systems, Inc., Minneapolis, MN) and Collagen type IIB (clone M2139, Thermo Fisher Scientific, Waltham, MA) and rabbit polyclonal primary antibodies for Lubricin (ab28484, Abcam, Cambridge, UK) and SOX9 (PA5-23383, Thermo Fisher Scientific, Waltham, MA).

    Techniques: Expressing, Immunohistochemical staining, Negative Control

    Effects of 5 different BMPs on the osteogenic differentiation of hMDSCs at day 28 (3D pellet culture). A. MicroCT 3D images showed that the mineralized pellets in 5 BMPs groups were larger than the CTL group. Scale bar=1mm. B. Quantification of mineralized pellet volume. All BMPs groups showed higher mineralized pellet volume compared to the CTL group. The mineralized pellet volume of the BMP2 group was significantly higher than the BMP6 and BMP7 groups. *P<0.05, ** P<0.01, ****, P<0.0001. C. Mineralized pellet density quantification. No statistical difference between BMP groups and the CTL group was found. D. Von Kossa staining. Brown-black color indicates mineralization. All pellets have regions peeled off due to high mineralization in the white region of pellets. E. Immunohistochemistry of osteocalcin for osteogenic pellets. Brown color indicates osteocalcin expression. The mineralized parts of the pellets demonstrate crystal purple color intermingled with osteocalcin brown staining. Scale bars=100μm.

    Journal: Biomaterials

    Article Title: The use of heparin/polycation coacervate sustain release system to compare the bone regenerative potentials of 5 BMPs using a critical sized calvarial bone defect model

    doi: 10.1016/j.biomaterials.2022.121708

    Figure Lengend Snippet: Effects of 5 different BMPs on the osteogenic differentiation of hMDSCs at day 28 (3D pellet culture). A. MicroCT 3D images showed that the mineralized pellets in 5 BMPs groups were larger than the CTL group. Scale bar=1mm. B. Quantification of mineralized pellet volume. All BMPs groups showed higher mineralized pellet volume compared to the CTL group. The mineralized pellet volume of the BMP2 group was significantly higher than the BMP6 and BMP7 groups. *P<0.05, ** P<0.01, ****, P<0.0001. C. Mineralized pellet density quantification. No statistical difference between BMP groups and the CTL group was found. D. Von Kossa staining. Brown-black color indicates mineralization. All pellets have regions peeled off due to high mineralization in the white region of pellets. E. Immunohistochemistry of osteocalcin for osteogenic pellets. Brown color indicates osteocalcin expression. The mineralized parts of the pellets demonstrate crystal purple color intermingled with osteocalcin brown staining. Scale bars=100μm.

    Article Snippet: Osteocalcin immunohistochemistry using a mouse anti-human osteocalcin primary antibody (MAB1419, 1:100, R&D Systems) was also performed, as previously described [ 27 ].

    Techniques: Staining, Immunohistochemistry, Expressing

    Levels of osteopontin (OP) secreted in the culture medium by osteoblasts cultured on the implants’ surface. A . in medium wih fetal calf serum (FCS), collected on day 8, 14, 21 and 28 (analysis with two-way ANOVA Bonferroni posttest *p < 0.05; **p < 0.01; ***p < 0.001); B . in serum-free medium (collected on day 21 and 28), in simple (OS) and in complex (OC) differentiation mediums (collected on day 35). Legend: cells cultivated in plastic dishes (ctrl), non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ).

    Journal: Journal of Biological Engineering

    Article Title: Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate

    doi: 10.1186/1754-1611-8-14

    Figure Lengend Snippet: Levels of osteopontin (OP) secreted in the culture medium by osteoblasts cultured on the implants’ surface. A . in medium wih fetal calf serum (FCS), collected on day 8, 14, 21 and 28 (analysis with two-way ANOVA Bonferroni posttest *p < 0.05; **p < 0.01; ***p < 0.001); B . in serum-free medium (collected on day 21 and 28), in simple (OS) and in complex (OC) differentiation mediums (collected on day 35). Legend: cells cultivated in plastic dishes (ctrl), non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ).

    Article Snippet: Osteopontin, osteocalcine, and collagen 1A1 (all mouse anti-human primary antibodies from Santa Cruz Biotechnologies) were diluted at a ratio of 1:50 in 1% BSA and incubated overnight at 4°C with the samples.

    Techniques: Cell Culture, Control

    Levels of osteopontin (OP) in cellular lysates of osteoblasts cultured on the titanium implants and measured on day 8 and 14 of cultivation. Legend: non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ), cells cultivated on plastic dishes (ctrl).

    Journal: Journal of Biological Engineering

    Article Title: Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate

    doi: 10.1186/1754-1611-8-14

    Figure Lengend Snippet: Levels of osteopontin (OP) in cellular lysates of osteoblasts cultured on the titanium implants and measured on day 8 and 14 of cultivation. Legend: non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ), cells cultivated on plastic dishes (ctrl).

    Article Snippet: Osteopontin, osteocalcine, and collagen 1A1 (all mouse anti-human primary antibodies from Santa Cruz Biotechnologies) were diluted at a ratio of 1:50 in 1% BSA and incubated overnight at 4°C with the samples.

    Techniques: Cell Culture, Control

    Immunocytochemical staining of the osteoblasts cultivated on the surface of the implants showing the expression of osteopontin (OP). Osteoblasts were stained with an anti OP-FITC monoclonal antibody on day 3, 14 and 21 days (magnification 100×). Legend: non-infiltrated control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ).

    Journal: Journal of Biological Engineering

    Article Title: Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate

    doi: 10.1186/1754-1611-8-14

    Figure Lengend Snippet: Immunocytochemical staining of the osteoblasts cultivated on the surface of the implants showing the expression of osteopontin (OP). Osteoblasts were stained with an anti OP-FITC monoclonal antibody on day 3, 14 and 21 days (magnification 100×). Legend: non-infiltrated control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ).

    Article Snippet: Osteopontin, osteocalcine, and collagen 1A1 (all mouse anti-human primary antibodies from Santa Cruz Biotechnologies) were diluted at a ratio of 1:50 in 1% BSA and incubated overnight at 4°C with the samples.

    Techniques: Staining, Expressing, Control

    Immunocytochemical staining of osteoblasts on the surface of the implants (noninfiltrated Ti, Ti infiltrated with HA and Ti infiltrated with SiO 2 ) showing the expression of actin F and osteopontin (OP). Osteoblasts were stained with anti OP-FITC monoclonal antibody, phalloidin TRITC (for actin F) and DAPI (for nuclei). The images were taken on day 14 and composed with Axiovision Release 4.6.3. image analysis software (magnification 200×). Legend: non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ).

    Journal: Journal of Biological Engineering

    Article Title: Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate

    doi: 10.1186/1754-1611-8-14

    Figure Lengend Snippet: Immunocytochemical staining of osteoblasts on the surface of the implants (noninfiltrated Ti, Ti infiltrated with HA and Ti infiltrated with SiO 2 ) showing the expression of actin F and osteopontin (OP). Osteoblasts were stained with anti OP-FITC monoclonal antibody, phalloidin TRITC (for actin F) and DAPI (for nuclei). The images were taken on day 14 and composed with Axiovision Release 4.6.3. image analysis software (magnification 200×). Legend: non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO 2 ).

    Article Snippet: Osteopontin, osteocalcine, and collagen 1A1 (all mouse anti-human primary antibodies from Santa Cruz Biotechnologies) were diluted at a ratio of 1:50 in 1% BSA and incubated overnight at 4°C with the samples.

    Techniques: Staining, Expressing, Software, Control